Human/Mouse/Rat Smad7 MAb (Clone 293039) (25 UG)【科瑞奥博】

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Human/Mouse/Rat Smad7 MAb (Clone 293039) (25 UG)

货号： MAB2029-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2029-SP
别名	CRCS3; FLJ16482; hSMAD7; MAD homolog 7; MAD homolog 8; MAD, mothers against decapentaplegic homolog 7 (Drosophila); MADH7; MADH7mothers against decapentaplegic homolog 7; MADH8; Mothers against decapentaplegic homolog 8; Mothers against decapentaplegic, drosophila, homolog of, 7; Mothers against DPP homolog 7; Mothers against DPP homolog 8; SMAD 7; SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7; SMAD, mothers against DPP homolog 7 (Drosophila); SMAD, mothers against DPP homolog 7; Smad7
全称	Mothers Against DPP Homolog 7
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human, mouse, and rat Smad7.
使用方法	Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4092 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The TGF?-SMAD3 pathway inhibits IL-1? induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration J Exp Clin Cancer Res, 2016;35(1):122. Species: Human Sample Type: Cell Lysates Application: WB TGF-Beta Induces Endometriotic Progression via a Non-Canonical, KLF11 Mediated Mechanism Endocrinology, 2016;0(0):en20161194. Species: Human Sample Type: Cell Lysates Application: WB Downregulation of the TGF-? pseudoreceptor BAMBI in non-small cell lung cancer enhances TGF-? signaling and invasion Cancer Res, 2016;0(0):. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5. Authors: Bardita C, Predescu D, Sha F, Patel M, Balaji G, Predescu S J Cell Sci, 2015;128(8):1528-41. Species: Mouse Sample Type: Cell Lysates Application: WB Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy. Authors: Mehuron T, Kumar A, Duarte L, Yamauchi J, Accorsi A, Girgenrath M Skelet Muscle, 2015;4(0):14. Species: Mouse Sample Type: Tissue Homogenates Application: WB Loss of N-Myc interactor promotes epithelial-mesenchymal transition by activation of TGF-beta/SMAD signaling. Authors: Devine D, Rostas J, Metge B, Das S, Mulekar M, Tucker J, Grizzle W, Buchsbaum D, Shevde L, Samant R Oncogene, 2014;33(20):2620-8. Species: Human Sample Type: Cell Lysates Application: WB microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition. Authors: Parikh A, Lee C, Joseph P, Marchini S, Baccarini A, Kolev V, Romualdi C, Fruscio R, Shah H, Wang F, Mullokandov G, Fishman D, DIncalci M, Rahaman J, Kalir T, Redline R, Brown B, Narla G, DiFeo A Nat Commun, 2014;5(0):2977. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Angiotensin II upregulates Kv1.5 expression through ROS-dependent transforming growth factor-beta1 and extracellular signal-regulated kinase 1/2 signalings in neonatal rat atrial myocytes. Authors: Lu G, Xu S, Peng L, Huang Z, Wang Y, Gao X Biochem Biophys Res Commun, 2014;454(3):410-416. Species: Rat Sample Type: Cell Lysate Application: WB Protons sensitize epithelial cells to mesenchymal transition. Authors: Wang M, Hada M, Saha J, Sridharan D, Pluth J, Cucinotta F PLoS ONE, 2012;7(7):e41249. Species: Human Sample Type: Cell Lysates Application: WB The TGF-ÃŽÂ²/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced intestinal injury independently of a Smad signaling pathway. Authors: Hneino M, Francois A, Buard V, Tarlet G, Abderrahmani R, Blirando K, Hoodless PA, Benderitter M, Milliat F PLoS ONE, 2012;7(5):e35672. Species: Human Sample Type: Cell Lysates Application: WB The integrative analysis of microRNA and mRNA expression in mouse uterus under delayed implantation and activation. Authors: Su RW, Lei W, Liu JL PLoS ONE, 2010;5(11):e15513. Species: Mouse Sample Type: Tissue Homogenates Application: WB Nuclear function of Smad7 promotes myogenesis. Authors: Miyake T, Alli NS, McDermott JC Mol. Cell. Biol., 2010;30(3):722-35. Species: Mouse Sample Type: Cell Lysate Application: WB 3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins. Authors: Seong HA, Jung H, Kim KT, Ha H J. Biol. Chem., 2007;282(16):12272-89. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Smad7 Gly320-Ser398 Accession # O15105
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Epithelial Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators ICC / IHC Images: Smad7 Intracellular Signaling Molecules in Angiogenesis Mesenchymal to Epithelial Transition Negative Regulators of EMT Neural Stem Cell Transcription Factors Smad Family Smad Transcription Factors TGF-beta Family TGF-beta Superfamily Modulators Transcription Factors in the Akt Pathway

参考图片

Smad7 in Human Liver Cancer Tissue. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human/Mouse/Rat Smad7 by Western Blot. Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Smad7 in Human Liver. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Smad7 by Simple Western^TM. Simple Western lane view shows lysates of Sf 21S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.