| 货号 | MAB1889-SP |
| 别名 | CMAP; CST7; Cystatin 7; cystatin F (leukocystatin); Cystatin-7; cystatin-F; Cystatin-like metastasis-associated protein; Leukocystatin |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Cystatin F in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin A, B, C, D, E/M, S, recombinant mouse (rm) Cystatin/Stefin Homolog, rhFetuin A, rhFetuin B, rhHPRG, rhKininogen, or rhKininostatin is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8530 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Cystatin F Gly20-His145 Accession # O76096 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a member of the cystatin superfamily (1‑3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards cathepsin L and legumain (1, 2, 5). Compared to other secreted cystatins including C, D, E/M, S, SA and SN, which contain two intra disulfide bonds, cystatin F has two extra Cys residues that may be involved in inter disulfide bonds. Indeed, rhCystatin F showed disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human cystatin F and glutathione S-transferase (2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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