货号 | MAB26051-SP |
别名 | 131320; GATA binding protein 3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3 |
反应种属 | Human |
应用 | Immunocytochemistry(8-25 µg/mL) |
目标/特异性 | Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with rhGATA-1, -2, -4, -5, or -6. |
使用方法 | Immunocytochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 2625 (Human); 14462 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cyclic regulation of T-Bet and GATA-3 in human endometrium. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human GATA-3 Pro135-Ser258 Accession # P23771 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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GATA‑3 in MCF-7 Human Cell Line. GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GATA‑3 Monoclonal Antibody (Catalog # MAB26051) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |