Human CD43 MAb (Clone 290111) (25 UG)【科瑞奥博】

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Human CD43 MAb (Clone 290111) (25 UG)

货号： MAB2038-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2038-SP
别名	CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human CD43. Stains human CD43-transfected cells but not irrelevant transfectants.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6693 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1alpha, IL-1beta, IL-12b, and CCL4 from differentiated HL-60 cells. Authors: Naegelen I, Plancon S, Nicot N, Kaoma T, Muller A, Vallar L, Tschirhart E, Brechard S J Leukoc Biol, 2015;97(3):557-71. Species: Human Sample Type: Whole Cells Application: IF
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0 mouse myeloma cell line transfected with human CD43 Ser20-Pro400 Accession # P16150
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CD43, also known as Leukosialin and Sialophorin and Ly-48 in mouse, is a 100-105 kDa type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells. Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific. CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion. Mature CD43 shows 49% amino (aa) sequence identity between human and mouse.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling B Cell Activation B Cell Markers Basophils Dendritic Cell Adhesion and Migration Eosinophils Granulocyte Markers Human CD Marker Products Leukocyte Receptors/Markers in Allergy and Asthma Monocyte Markers Mouse Tissue-Resident Macrophage Markers Myeloid-derived Suppressor Cells (MDSC) Neutrophils Special Glycoproteins T Cell Migration/Adhesion

参考图片

Detection of CD43 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) or (B) Mouse IgG_2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody. View our protocol for Staining Membrane-associated Proteins.

CD43 in Human PBMCs. CD43 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD43 Monoclonal Antibody (Catalog # MAB2038) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.