| 货号 | MAB28952-SP |
| 别名 | EC 3.4.24.63; endopeptidase-2; MEP1B; meprin A subunit beta; meprin A, beta; Meprin B; N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta; PABA peptide hydrolase; PPH beta |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human or mouse Pro Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4225 (Human); 17288 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human embryonic kidney cell line HEK293-derived recombinant human Meprin beta Subunit/MEP1B Thr23-Ser593 Accession # Q16820 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of the human Meprin beta subunit precursor consists of a signal peptide (aa 1 - 21), a pro region (aa 22 - 61), and a mature chain (aa 62 - 701) containing the following domains: catalytic (aa 62 - 259), MAM (aa 260 - 429), MATH (aa 430 - 585), EGF-like (aa 604 - 644), transmembrane (aa 653 - 673), and cytoplasmic (aa 674 - 701). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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