货号 | MAB2697-SP |
别名 | DNA-binding factor KBF1; EBP-1; NF-kappa-B; NFKB1; NF-kB1; NFKB-p105; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; p105 | 全称 | Nuclear Factor Kappa-B, Subunit 1 |
反应种属 | Human/Mouse |
应用 | Western Blot(0.5 µg/mL) |
目标/特异性 | Detect endogenous human NF kappa B1 in Western blots. |
使用方法 | Western Blot: 0.5 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 4790 (Human); 18033 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD40 promotion of amyloid beta production occurs via the NF-kappaB pathway. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant human NF kappa B1 Pro317-Asp437 Accession # P19838 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Nuclear Factor kappa B1 (NF kappa B1 or NF kappa B p50) is a member of the NF kappa B/Rel family of transcription factors. NF kappa B1 dimerizes with other members of the NF kappa B/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human NF kappa B1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse NF kappa B1 Monoclonal Antibody (Catalog # MAB2697) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NF kappa B1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |