Human TNF-alpha MAb (Clone 28401) (25 UG)【科瑞奥博】

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Human TNF-alpha MAb (Clone 28401) (25 UG)

货号： MAB610-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB610-SP
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
目标/特异性	Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF‑ beta, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant porcine TNF‑ alpha is observed.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.01-0.04 µg/mL in the presence of 0.75 ng/mL Recombinant Human TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 28401
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lysine fatty acylation promotes lysosomal targeting of TNF-? Authors: H Jiang, X Zhang, H Lin Sci Rep, 2016;6(0):24371. Species: Human Sample Type: Whole Cell Application: IHC Fresh Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcgammaR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment. Authors: Matt P, Lindqvist U, Kleinau S PLoS ONE, 2015;10(9):e0137474. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Capture Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Authors: Todd J, Simpson P, Estis J, Torres V, Wub A Cytokine, 2013;64(3):660-5. Species: Human Sample Type: Plasma Application: ELISA Development A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Authors: Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR Cancer Res., 2012;72(1):66-75. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Protection from RNA and DNA viruses by IL-32. Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF J. Immunol., 2011;186(7):4110-8. Species: Human Sample Type: Cell Culture Supernates Application: Electrochemiluminescence C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY Diabetologia, 2011;54(10):2713-23. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK J. Immunol., 2008;180(4):2553-61. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis. Authors: Colucci S, Brunetti G, Cantatore FP, Oranger A, Mori G, Quarta L, Cirulli N, Mancini L, Corrado A, Grassi FR, Grano M J. Pathol., 2007;212(1):47-55. Species: Human Sample Type: Whole Cells Application: Neut Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Clin. Chem., 2007;53(11):2010-2. Species: Human Sample Type: Plasma Application: ELISA Development Interleukin-10 modulates pro-apoptotic effects of TNF-alpha in human articular chondrocytes in vitro. Authors: John T, Muller RD, Oberholzer A, Zreiqat H, Kohl B, Ertel W, Hostmann A, Tschoeke SK, Schulze-Tanzil G Cytokine, 2007;40(3):226-34. Species: Human Sample Type: Whole Cells Application: ICC Ultrasensitive flow-based immunoassays using single-molecule counting. Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Clin. Chem., 2007;53(11):1990-5. Species: Human Sample Type: Plasma Application: ELISA Development HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P AIDS, 2007;21(10):1263-72. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose. Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M J. Clin. Oncol., 2007;25(29):4542-9. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Selective regulation of IL-10 signaling and function by zymosan. Authors: Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB J. Immunol., 2006;176(8):4785-92. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Increased ENA-78 in the follicular fluid of patients with endometriosis. Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA Acta Obstet Gynecol Scand, 2006;85(3):336-42. Species: Human Sample Type: Follicular Fluid Application: ELISA Development Sequential induction of angiogenic growth factors by TNF-alpha in choroidal endothelial cells. Authors: Hangai M, He S, Hoffmann S, Lim JI, Ryan SJ, Hinton DR J. Neuroimmunol., 2005;171(1):45-56. Species: Human Sample Type: Whole Tissue Application: IHC Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Authors: Phillips TM Electrophoresis, 2004;25(10):1652-9. Species: Human Sample Type: Serum Application: Affinity Chromatography IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. Authors: Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB J. Immunol., 2004;172(10):6476-81. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. Authors: Copeland S, Siddiqui J, Remick D J. Immunol. Methods, 2004;284(1):99-106. Species: Human Sample Type: Cell Culture Supernates Application: Array Development True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha? Authors: Buch MH, Conaghan PG, Quinn MA, Bingham SJ, Veale D, Emery P Ann. Rheum. Dis., 2004;63(10):1344-6. Species: Human Sample Type: Whole Tissue Application: IHC Frozen trans fatty acids and systemic inflammation in heart failure. Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC Am. J. Clin. Nutr., 2004;80(6):1521-5. Species: Human Sample Type: Plasma Application: ELISA Development Reprogramming of IL-10 activity and signaling by IFN-gamma. Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB J. Immunol., 2003;171(10):5034-41. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF-alpha, designated TNFSF2, is a trimeric glycoprotein active in bothmembrane bound and secreted forms. TNF-alpha is produced by severallymphoid cells as well as by astrocytes, endothelial cells, and smoothmuscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtuallyall cell types where it triggers the activation of multiple signaltransduction pathways and modulates the expression of a wide variety ofgenes.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cancer Biomarkers Conventional/Classical Dendritic Cells Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction gamma delta T Cells ICC / IHC Images: TNF-alpha Inflammatory Mediators Inflammatory/Monocyte-derived Dendritic Cells Interactive Pathway: Adipocytokines & Insulin Signaling Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Natural Killer T (NKT) Cells Neuroinflammation Obesity Plasmacytoid Dendritic Cells Preeclampsia Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members T Cell Cytokine Signaling Technical Notes: R&D Systems Is With You Every Step of the Way - Analyzing Proteins of Interest Th1 Cells Th22 Cells TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody. Recombinant Human TNF‑ alpha (Catalog #
210‑TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF‑ alpha Monoclonal Antibody (Catalog # MAB610). The ND₅₀ is typically
0.01‑0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF‑ alpha in Human PBMCs. TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human TNF‑ alpha Monoclonal Antibody (Catalog # MAB610) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.