Human/Mouse IL-12/IL-35 p35 MAb (Clone 27537) (25 UG)【科瑞奥博】

网站首页公司简介产品中心技术服务新闻动态帮助中心访客留言联系我们

产品中心

北京博奥森生物技术有限公司: 一抗; 标记一抗; 二抗; 标记二抗; 蛋白质与多肽; 标记蛋白质与多肽; 正常动物血清及免疫球蛋白; 试剂盒; 常用试剂; 亲和层析柱; 配套试剂

北京索莱宝有限公司: 生化试剂; 分子生物学; 蛋白与免疫; 细胞生物学; 标准品; 通用耗材; 培养基; 通用缓冲液; 染料/染色剂; 层析填料树脂; 生化试剂盒; 发酵专用产品; 诊断试剂

上海科兴生物科技有限公司: ELISA试剂盒; 生化试剂; 血浆; 血清; 细胞; 标准品; 抗体; 裂解血; 培养基

技术服务

多克隆抗体制备

单克隆抗体制备

多肽合成服务

重组蛋白表达和纯化服务

蛋白纯化服务

标记服务

产品中心

当前位置：首页>产品中心

Human/Mouse IL-12/IL-35 p35 MAb (Clone 27537) (25 UG)

货号： MAB1570-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1570-SP
简介	**This product is in the procress of being discontinued
别名	IL-12A
全称	Interleukin 12 p35
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
使用方法	Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Gene expression and antiviral activity of interleukin-35 in response to influenza A virus infection Authors: Li Wang J Biol Chem, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R Proc Natl Acad Sci U S A, 2015;112(9):E957-65. Species: Mouse Sample Type: Whole Tissue Application: Bioassay Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region. Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S J Immunol, 2015;194(1):113-24. Species: Human Sample Type: Whole Cells Application: Neut Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo. Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M Nat Commun, 2015;6(0):6353. Species: Mouse Sample Type: Whole Cells Application: Flow Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D J Immunol, 2014;192(2):804-16. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D J Immunol, 2012;189(12):5590-601. Species: Human Sample Type: whole cells Application: Neut Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Authors: Jindra C Eur J Immunol, 2010;40(2):321-9. Species: Human Sample Type: Cell Culture Supernates Application: IP Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A J. Allergy Clin. Immunol., 2009;123(1):239-48. Species: Human Sample Type: Whole Cells Application: Flow Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Eur. J. Immunol., 2008;38(4):1012-23. Species: Human Sample Type: Whole Cells Application: Flow Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination. Authors: Nagarajan S, Selvaraj P Vaccine, 2005;24(13):2264-74. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 iscomprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺regulatory T cells and may function as an inhibitory cytokine to suppress Tcell proliferation (2). Human FoxP3⁺ Tregs do not constitutivelyexpress IL-35 (3), but expression may be induced by activated dendritic cells(4).
运输条件	Blue Ice
存放说明	4℃
参考文献	Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248. Collison, L.W. et al. (2007) Nature 450:566. Bardel,E.et al. (2008) J. Immunol. 181:6898. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.