Human/Mouse Pax3 MAb (Clone 274212) (25 UG)【科瑞奥博】

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Human/Mouse Pax3 MAb (Clone 274212) (25 UG)

货号： MAB2457-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2457-SP
别名	CDHS; HUP2; HUP2MGC120384; MGC120381; MGC120382; MGC120383; MGC134778; paired box 3; paired box gene 3 (Waardenburg syndrome 1); paired box homeotic gene 3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; Waardenburg syndrome 1; WS1; WS3
全称	Paired Box Gene 3 [Waardenburg Syndrome 1]
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human and mouse Pax3 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5077 (Human); 18505 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Functional dissection of Pax3 in paraxial mesoderm development and myogenesis. Authors: Magli A, Schnettler E, Rinaldi F, Bremer P, Perlingeiro R Stem Cells, 2013;31(1):59-70. Species: Mouse Sample Type: Cell Lysate Application: WB Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells. Authors: Awaya, Tomonari, Kato, Takeo, Mizuno, Yuta, Chang, Hsi, Niwa, Akira, Umeda, Katsutsu, Nakahata, Tatsutos, Heike, Toshio PLoS ONE, 2012;7(12):e51638. Species: Human Sample Type: Whole Cells Application: ICC MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells. Authors: Gianakopoulos PJ, Mehta V, Voronova A, Cao Y, Yao Z, Coutu J, Wang X, Waddington MS, Tapscott SJ, Skerjanc IS J. Biol. Chem., 2011;286(4):2517-25. Species: Mouse Sample Type: Cell Lysates Application: WB Pax3 activation promotes the differentiation of mesenchymal stem cells toward the myogenic lineage. Authors: Gang EJ, Bosnakovski D, Simsek T, To K, Perlingeiro RC Exp. Cell Res., 2008;314(8):1721-33. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Pax3 (isoform Pax3a) Met1-Ser215 Accession # NP_000429
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Pax3 belongs to the family of paired box transcription factors. Paxfamily proteins typically contain a paired box domain and a paired-typehomeodomain. These transcription factors play critical roles duringfetal development. Human and mouse Pax3 share 98% amino acid sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cancer Stem Cell Transcription Factors Epithelial Stem Cell Transcription Factors ICC / IHC Images: Pax3 Mesenchymal Stem Cell Transcription Factors Muscle Stem Cell Markers Myogenesis Markers Myogenic Precursor Markers Neural Crest Cell Markers Neural Stem Cell Markers Neural Stem Cell Transcription Factors Paired Box Transcription Factors Somite Markers Transcription Factors in the Akt Pathway

参考图片

Pax3 in B16‑F1 Mouse Cell Line. Pax3 was detected in immersion fixed B16‑F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.