| 货号 | MAB2506-SP |
| 别名 | EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta isoform | 全称 | Glycogen Synthase Kinase 3 beta |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human, mouse, and rat GSK-3 beta in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha. |
| 使用方法 | Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2932 (Human); 56637 (Mouse); 84027 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. beta-catenin mediates insulin-like growth factor-I actions to promote cyclin D1 mRNA expression, cell proliferation and survival in oligodendroglial cultures. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human GSK-3 beta Met1-Thr420 Accession # P49841 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat GSK‑3 beta by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat GSK‑3 beta Monoclonal Antibody (Catalog # MAB2506) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human GSK-3 alpha and Recombinant Human Active GSK‑3 beta (Catalog # 2506-KS) (20 ng/lane) were included. A specific band for GSK-3 beta was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
