| 货号 | MAB25061-SP |
| 别名 | EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta isoform | 全称 | Glycogen Synthase Kinase 3 beta |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human GSK-3 beta. |
| 使用方法 | Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2932 (Human); 56637 (Mouse); 84027 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Discovery of novel AKT inhibitors with enhanced anti-tumor effects in combination with the MEK inhibitor. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human GSK-3 beta Met1-Thr420 Accession # P49841 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved inenergy metabolism, body pattern formation, and neuronal cell development. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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GSK‑3 beta in MCF-7 Human Cell Line. GSK‑3 beta was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GSK‑3 beta Monoclonal Antibody (Catalog # MAB25061) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
