| 货号 | MAB1939-SP |
| 别名 | DKFZp779M0222; EC 3.4.21; EC 3.4.21.7; plasmin; plasminogen; Plg |
| 反应种属 | Human |
| 应用 | Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human PLG, human Plasmin, and the catalytic domain in direct ELISAs and Western blots. |
| 使用方法 | Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Human Plasminogen (1 µg/mL, Catalog # 1939-SE) cleavage of the fluorogenic peptide substrate Suc-AFK-Amc (100 µM). The Neutralization Dose (ND50) is typically 1 µg/mL. |
| 来源 | Monoclonal Mouse IgG1 Clone # 270409 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5340 (Human); 18815 (Mouse); 85253 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A novel role for plasmin-mediated degradation of opsonizing antibody in the evasion of host immunity by virulent, but not attenuated, Francisella tularensis. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human plasma-derived Plasminogen Glu20-Asn810 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation, and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20-810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Neutralization of Plasminogen Activity by Human Plasminogen Antibody. The cleavage of Suc-AFK-AMC (100 µM) by Human Plasminogen (1 µg/mL, Catalog # 1939-SE) is measured after preincubation with increasing concentrations of Mouse Anti-Human Plasminogen Monoclonal Antibody (Catalog # MAB1939). The ND50 is typically 1 µg/mL. |
