| 货号 | MAB2214-SP |
| 别名 | activator protein 1; AP-1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; G0S3; G0S3G0/G1 switch regulatory protein 3; GOS3; GOSB; MGC42291; oncogene FOS-B; protein fosB | 全称 | FBJ Murine Osteosarcoma Viral Oncogene Homolog B |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human and mouse FosB in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2354 (Human); 14282 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human FosB Met1-Leu338 Accession # P53539 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | FosB (FBJ murine osteoscarcoma viral oncogene homolog B) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. AP-1 activity is important for gene transcription in a wide variety of cellular processes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse FosB/G0S3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with XXX TPA for 2 hours and NIH‑3T3 mouse embryonic fibroblast cell line untreated or treated with serum for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse FosB/G0S3 Monoclonal Antibody (Catalog # MAB2214), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FosB/G0S3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
