Human/Mouse PDX-1/IPF1 MAb (Clone 267712) (25 UG)【科瑞奥博】

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Human/Mouse PDX-1/IPF1 MAb (Clone 267712) (25 UG)

货号： MAB2419-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2419-SP
别名	Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1
全称	Pancreas/Duodenum Homeobox-1
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human and mouse PDX-1 in Western blots.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3651 (Human); 18609 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells Authors: P Saxena, BC Heng, P Bai, M Folcher, H Zulewski, M Fussenegge Nat Commun, 2016;7(0):11247. Species: Human Sample Type: Whole Cells Application: IHC - PFA fixed Redifferentiation of adult human beta cells expanded in vitro by inhibition of the WNT pathway. Authors: Lenz A, Toren-Haritan G, Efrat S PLoS ONE, 2014;9(11):e112914. Species: Human Sample Type: Whole Cells Application: ICC Peroxisome proliferator-activated receptor gamma activation restores islet function in diabetic mice through reduction of endoplasmic reticulum stress and maintenance of euchromatin structure. Authors: Evans-Molina C, Robbins RD, Kono T, Tersey SA, Vestermark GL, Nunemaker CS, Garmey JC, Deering TG, Keller SR, Maier B, Mirmira RG Mol. Cell. Biol., 2009;29(8):2053-67. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells. Authors: Li Y, Zhang R, Qiao H, Zhang H, Wang Y, Yuan H, Liu Q, Liu D, Chen L, Pei X J. Cell. Physiol., 2007;211(1):36-44. Species: Human Sample Type: Whole Cells Application: ICC Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells. Authors: Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK Biochem. Biophys. Res. Commun., 2007;366(1):129-34. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human PDX-1 Ala91-Arg283 Accession # P52945
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomaintranscription factor essential for pancreatic development and maturepancreatic beta cell function.
运输条件	Blue Ice
存放说明	4℃
参考文献	Diabetes Glucose Homeostasis Hepatic and Pancreatic Progenitor Cell Transcription Factors Homeodomain (Hox) Transcription Factors Human Pluripotent Stem Cell Antibody Array ICC / IHC Images: PDX-1/IPF1 Mesenchymal Stem Cell Transcription Factors Pancreatic Endoderm Pancreatic Endoderm Cell Markers Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of Mouse PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX‑1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX‑1/IPF1 was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.