货号 | MAB2759-SP |
别名 | ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; TOLL; toll-like receptor 4 | 全称 | Toll-like Receptor 4 |
反应种属 | Mouse |
应用 | Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
目标/特异性 | Detects mouse TLR4 in direct ELISAs. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4. |
使用方法 | Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 7099 (Human); 21898 (Mouse); 29260 (Rat) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Adjuvant effect of killed Propionibacterium acnes on mouse peritoneal B-1 lymphocytes and their early phagocyte differentiation. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TLR4 Asn26-Lys629 Accession # Q9QUK6 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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TLR4 in RAW 264.7 Mouse Monocyte/Macrophage Cell Line. TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line using Rat Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB2759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |