货号 | MAB2405-SP |
别名 | CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA |
反应种属 | Mouse |
应用 | Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/106cells) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL) |
目标/特异性 | Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 3, rmIntegrin beta 4, or rmIntegrin beta 6 is observed. |
使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/106cells Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3688 (Human); 16412 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Crawling Phagocytes Recruited in the Brain Vasculature after Pertussis Toxin Exposure through IL6, ICAM1 and ITGalphaM. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29 Gln21-Asp728 Accession # P09055 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Integrin beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog #F0105B). | |
Integrin beta 1/CD29 in L‑929 Mouse Cell Line. Integrin beta 1/CD29 was detected in immersion fixed L‑929 mouse fibroblast cell line using Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |