Porcine IL-10 MAb (Clone 262715) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Porcine IL-10 MAb (Clone 262715) (25 UG)

货号： MAB6932-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB6932-SP
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Porcine
应用	Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects porcine IL-10 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant canine IL-10, recombinant feline IL-10, recombinant human IL-10, and recombinant viral IL-10 is observed and no cross-reactivity with recombinant cotton rat IL-10, recombinant equine IL-10, recombinant mouse IL-10, recombinant rat IL-10, or recombinant human IL-22 is observed.
使用方法	Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. Authors: Nadworny PL, Wang J, Tredget EE J Inflamm (Lond), 2010;7(0):13. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant porcine IL-10 Ser19-Asn175 Accession # Q29055
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1⁺(CD5⁺) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 shares 71% and 78% amino acid sequence identity with mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.
运输条件	Blue Ice
存放说明	4℃
参考文献	Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

参考图片

IL‑10 in Porcine PBMCs.
IL‑10 was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Mouse Anti-Porcine IL‑10 Monoclonal Antibody (Catalog # MAB6932) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.