Mouse CCL6/C10 MAb (Clone 262016) (25 UG)【科瑞奥博】

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Mouse CCL6/C10 MAb (Clone 262016) (25 UG)

货号： MAB487-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB487-SP
别名	C10; MRP-1; SCYA6
反应种属	Mouse
应用	Western Blot(1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse CCL6/C10 in Western blots. In Western blots, nocross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14,15, 16, 17, 18, 19, 21, 22, 23, 24, 25, 28, recombinant mouse CCL3, 4, 9, 11,12, 19, 20, 21, 22, 24, 25, 28, or recombinant rat CCL20 is observed.
使用方法	Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize CCL6/C10-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Mouse CCL6/C10.
来源	Monoclonal Rat IgG_2B Clone # 262016
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	20305 (Mouse); 287910 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa. Authors: Kotarsky K, Sitnik KM, Stenstad H, Kotarsky H, Schmidtchen A, Koslowski M, Wehkamp J, Agace WW Mucosal Immunol, 2010;3(1):40-8. Species: Mouse Sample Type: Luminal Wash Application: ELISA Development Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CCL6/C10 Gly22-Ala116 Accession # P27784
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL6, also known as C10 and SCYA6, is a beta chemokine with an extended N-terminus. CCL6 expression is induced by GM-CSF stimulation of monocytes and neutrophils and IL-4 stimulation of macrophages. CCL6 functions as a monocyte and macrophage chemoattractant.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Inflammatory Mediators

参考图片

Chemotaxis Induced by CCL6/C10 and Neutralization by Mouse CCL6/C10 Antibody. Recombinant Mouse CCL6/C10 (Catalog # 487-C) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL6/C10 (1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB487). The ND₅₀ is typically 6‑24 µg/mL.