Human Dectin-1/CLEC7A MAb (Clone 259931) (25 UG)【科瑞奥博】

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Human Dectin-1/CLEC7A MAb (Clone 259931) (25 UG)

货号： MAB1859-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1859-SP
简介	Clone 259931 was used by HLDA to establish CD designation
别名	Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding. Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	64581 (Human); 56644 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects. Authors: Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T J Immunol, 2016;196(3):1272-83. Species: Human Sample Type: Whole Cells Application: Neut TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response. Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J Eur J Immunol, 2015;46(2):455-63. Species: Human Sample Type: Whole Cells Application: Neut Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production. Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A PLoS ONE, 2015;10(3):e0120948. Species: Human Sample Type: Whole Cells Application: Blocking Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization. Authors: Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S J Immunol, 2015;195(4):1723-31. Species: Human Sample Type: Whole Cells Application: Neut Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk. Authors: Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I Eur J Immunol, 2014;44(12):3729-40. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli. Authors: Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ Eur. J. Immunol., 2012;42(6):1512-22. Species: Human Sample Type: Whole Cells Application: Flow MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production. Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y PLoS ONE, 2012;7(12):e50589. Species: Human Sample Type: Whole Cells Application: Neut Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1beta via a noncanonical caspase-8 inflammasome. Authors: Gringhuis SI, Kaptein TM, Wevers BA, Theelen B, van der Vlist M, Boekhout T, Geijtenbeek TB Nat. Immunol., 2012;13(3):246-54. Species: Human Sample Type: Whole Cells Application: Neut Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Immunol. Lett., 2011;134(2):167-73. Species: Human Sample Type: Whole Cells Application: Flow Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes. Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK J. Leukoc. Biol., 2011;90(3):599-611. Species: Human Sample Type: Cell Lysates Application: IP Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2. Authors: Gringhuis SI, Wevers BA, Kaptein TM, van Capel TM, Theelen B, Boekhout T, de Jong EC, Geijtenbeek TB PLoS Pathog., 2011;7(1):e1001259. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A Thr66-Met201 Accession # NP_072092
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4⁺ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.
运输条件	Blue Ice
存放说明	4℃
参考文献	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288. Yokota, K. et al. (2001) Gene 272:51. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771. Gantner, B.N. et al. (2005) EMBO J. 24:1277. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119. Yadav, M. and J.S. Schorey (2006) Blood 108:3168. Suram, S. et al. (2006) J. Biol. Chem. 281:5506. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154. Saijo, S. et al. (2007) Nat. Immunol. 8:39. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

参考图片

Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin‑1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin‑1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.