货号 | MAB1765-SP |
别名 | dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; DKK-1; hDkk-1; SKdickkopf-1 like | 全称 | Dickkopf-1 |
反应种属 | Mouse |
应用 | Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) |
目标/特异性 | Detects mouse Dkk-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human Dkk-1, -3, or -4. |
使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 22943 (Human); 13380 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Thiazolidinediones increase the wingless-type MMTV integration site family (WNT) inhibitor Dickkopf-1 in adipocytes: a link with osteogenesis. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Dkk-1 Ser30-His272 Accession # O54908 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Dkk‑1 in Mouse Embryo. Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c) using Mouse Dkk‑1 Monoclonal Antibody (Catalog # MAB1765) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |