| 货号 | MAB24441-SP |
| 别名 | cardiac-specific homeo box; cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX1; CSXFLJ97195; FLJ52202; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5 (Drosophila); NKX2.5FLJ97166; NKX2-5; NKX2E; NKX2EFLJ97197; NKX4-1; tinman paralog | 全称 | NK2 Homeobox 5 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects endogenous human NKX2.5 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1482 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human NKX2.5 Gln24-Arg137 Accession # P52952 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart. NKX2.5 protein contains a central single DNA binding domain flanked by two Ala/Pro rich domains. Sumoylation of NKX2.5 at Lys-51 enhances its DNA binding and transcriptional activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human NKX2.5 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441) followed by HRP-conjugated Anti‑Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human NKX2.5 by Simple WesternTM. Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line and iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for NKX2.5 at approximately 48 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB24441). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
