| 货号 | MAB3444-SP |
| 别名 | Atoh5; BHLHA7; bHLHa7NGN-3; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; neurogenin 3; neurogenin-3; NGN3; Protein atonal homolog 5; Relax |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Neurogenin-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50674 (Human); 11925 (Mouse); 60329 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Neurogenin-3 Met1-Leu214 Accession # Q9Y4Z2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Neurogenin-3 (NGN3) is a 23 kDa developmentally expressed nuclear protein of the basic helix-loop-helix (bHLH) family, class B subfamily. bHLH proteins are transcription factors that form dimers to bind DNA. Neurogenin-3 expression is required for all endocrine lineage differentiation in the pancreas, including formation of islet beta cells. Neurogenin-3 is also neurogenic, controlling the number of dendrites developed by hippocampal neurons. Human Neurogenin-3 shows 76% and 77% amino acid identity with mouse and rat Neurogenin-3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Neurogenin‑3 by Western Blot. Western blot shows lysates of human heart tissue, human placenta tissue, human prostate tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Neurogenin‑3 Monoclonal Antibody (Catalog # MAB3444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Neurogenin‑3 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
