Mouse IL-23 R MAb (Clone 258010) (25 UG)【科瑞奥博】

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Mouse IL-23 R MAb (Clone 258010) (25 UG)

货号： MAB1686-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1686-SP
别名	IBD17; IL-23 receptor; IL-23R; interleukin 23 receptor; interleukin-23 receptor
全称	Interleukin 23 Receptor
反应种属	Mouse
应用	Western Blot(1 µg/mL)
目标/特异性	Detects mouse IL-23 R in Western blots. In Western blots, this antibody does not cross-react with recombinant human IL-23 R.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑23-induced IL‑17 secretion in mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑23 and 10 ng/mL Recombinant Mouse IL‑2.
来源	Monoclonal Rat IgG_2B Clone # 258010
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	149233 (Human); 209590 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection. Authors: Kumar P, Thakar M, Ouyang W, Malarkannan S Mucosal Immunol, 2013;6(1):69-82. Species: Mouse Sample Type: whole cells Application: Flow Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy. Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L J. Exp. Med., 2011;208(3):491-503. Species: Mouse Sample Type: Whole Cells Application: Neut A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses. Authors: Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL Nat. Med., 2009;15(9):1016-22. Species: Mouse Sample Type: In Vivo Application: In vivo Cutting edge: An in vivo requirement for STAT3 signaling in TH17 development and TH17-dependent autoimmunity. Authors: Harris TJ, Grosso JF, Yen HR, Xin H, Kortylewski M, Albesiano E, Hipkiss EL, Getnet D, Goldberg MV, Maris CH, Housseau F, Yu H, Pardoll DM, Drake CG J. Immunol., 2007;179(7):4313-7. Species: Mouse Sample Type: In Vivo Application: In vivo Resident Vdelta1+ gammadelta T cells control early infiltration of neutrophils after Escherichia coli infection via IL-17 production. Authors: Shibata K, Yamada H, Hara H, Kishihara K, Yoshikai Y J. Immunol., 2007;178(7):4466-72. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R Gly24-Asp372 Accession # Q8K4B4
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4⁺CD45RB^low memory T cells but at much lower levels by mouse CD4⁺CD45RB^high cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.
运输条件	Blue Ice
存放说明	4℃
参考文献	Oppmann, B. et al. (2000) Immunity 13:715. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49. Parham, C. et al. (2002) J. Immunol. 168:5448. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

参考图片

IL‑17 Secretion Induced by
IL‑23 and Neutralization by Mouse IL‑23 R Antibody. In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402-ML), Recombinant Mouse IL‑23 (Catalog # 1887-ML) stimulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‑17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL‑17 secretion elicited by Recombinant Mouse IL‑23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑23 R Monoclonal Antibody (Catalog # MAB1686). The ND₅₀ is typically 0.1-0.4 µg/mL.