| 货号 | MAB2317-SP |
| 别名 | 145kD; EC 3.1.3; hp51CNMGC142142; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145kDa; INPP5D; MGC104855; SHIPMGC142140 | 全称 | SH2-containing Inositol Phosphatase |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat SHIP in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3635 (Human); 16331 (Mouse); 54259 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse SHIP Glu874-Thr941 Accession # Q9ES52 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SHIP by Western Blot. Western blot shows lysates of TF‑1 human erythroleukemic cell line. Gels were loaded with 10 µg (lane 1), 20 µg (lane 2), and 40 µg (lane 3). PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for SHIP was detected at approximately ??? kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. | |
SHIP in Mouse Splenocytes. SHIP was detected in immersion fixed mouse splenocytes using Rat Anti‑Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
