| 货号 | MAB2095-SP |
| 别名 | KIAA1479FLJ11598; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D; Sema6D; semaphorin-6D |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Semaphorin 6D in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) Semaphorin 3A and recombinant mouse (rm) Semaphorin 3C is observed and no cross‑reactivity with rhSemaphorin 3B, 6A, 6B, 6C, 7A, rmSemaphorin 3E, 3F, or 6D is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 80031 (Human); 214968 (Mouse); 311384 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Semaphorin 6D Ser22-His660 Accession # Q8NFY4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Semaphorin 6D (Sema6D) is a ~130-135 kDa member of the Semaphorin family of axon guidance molecules (1-3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that share ~40% amino acid (aa) identity and exhibit neuropilin-independent binding to specific plexin A receptors (1, 2). Sema6D is expressed in the cardiac tube and within the brain and spinal cord during development (2-5). It shows broad expression postnatally, including neurons, lymphocytes, dendritic cells and osteoclasts (5-8). Brain, kidney, placenta, and cardiac and skeletal muscles show highest mRNA expression (2, 5). The primary human Sema6D isoform (1073 aa) includes a 21 aa signal sequence, a 642 aa extracellular domain (ECD) including Sema and PSI domains, a 21 aa transmembrane sequence and a 390 aa cytoplasmic portion. The ECD of this isoform shares 96%, 96%, 98%, 98% and 97% aa identity with corresponding mouse, rat, bovine, equine and canine sequences, respectively. Alternate splicing creates isoforms of 1054, 1030, 1017, 1011 and 998 that lack sequences between aa 570 and 644 and/or contain a 13 aa insert after aa 549; all variations affect the PSI domain (2, 5). An isoform of 476 aa is truncated after the Sema domain (2). All Sema6D isoforms are present in the brain, but are differentially expressed elsewhere (2, 5). In the developing brain, both Sema6D and Sema6C are co-expressed with Plexin A1 and interact differentially to guide proprioceptive peripheral neurons by repulsion (4). Sema6D is essential for morphogenesis of the cardiac ventricle in the chick, signaling both forward through Plexin A1 and in reverse through Sema6D (3, 9). Sema6D mediates survival and anchorage-independent growth of malignant pleural mesothelioma (10) It is active in immune responses by Sema6D+ T cell stimulation of dendritic cells, and in bone homeostasis by engaging osteoclast Plexin A1 (6-8). In all these settings, Sema6D acts through a complex of Plexin A1 with receptor tyrosine kinases such as VEGF R2 (3, 6-10). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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