Human/Mouse Pin1 MAb (Clone 257417) (25 UG)【科瑞奥博】

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Human/Mouse Pin1 MAb (Clone 257417) (25 UG)

货号： MAB2294-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2294-SP
别名	dod; EC 5.2.1.8; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; peptidyl-prolyl cis/trans isomerase, NIMA-interacting; peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1; Rotamase Pin1; UBL5
全称	Protein [Peptidyl-prolyl cis/trans Isomerase] NIMA-interacting 1
反应种属	Human/Mouse
应用	Western Blot(0.5 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunoprecipitation(2-3 µg/500 µg cell lysate)
目标/特异性	Detects human and mouse Pin1 in Western blots.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 2-3 µg/500 µg cell lysate
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5300 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The peptidyl-prolyl isomerase Pin1 up-regulation and proapoptotic function in dopaminergic neurons: relevance to the pathogenesis of Parkinson disease. Authors: Ghosh A, Saminathan H, Kanthasamy A, Anantharam V, Jin H, Sondarva G, Harischandra D, Qian Z, Rana A, Kanthasamy A J Biol Chem, 2013;288(30):21955-71. Species: Human Sample Type: Tissue Homogenates Application: WB A distinct role for Pin1 in the induction and maintenance of pluripotency. Authors: Nishi M, Akutsu H, Masui S J. Biol. Chem., 2011;286(13):11593-603. Species: Human Sample Type: Cell Lysates Application: IP Pin1 regulates TGF-beta1 production by activated human and murine eosinophils and contributes to allergic lung fibrosis. Authors: Shen ZJ, Esnault S, Rosenthal LA, Szakaly RJ, Sorkness RL, Westmark PR, Sandor M, Malter JS J. Clin. Invest., 2008;118(2):479-90. Species: Mouse Sample Type: Cell Lysates Application: WB Protein Never in Mitosis Gene A Interacting-1 (PIN1) regulates degradation of inducible nitric oxide synthase in endothelial cells. Authors: Liu T, Huang Y, Likhotvorik RI, Keshvara L, Hoyt DG Am. J. Physiol., Cell Physiol., 2008;295(3):C819-27. Species: Mouse Sample Type: Cell Lysates Application: IP The peptidyl-isomerase Pin1 regulates p27kip1 expression through inhibition of Forkhead box O tumor suppressors. Authors: Brenkman AB, de Keizer PL, van den Broek NJ, van der Groep P, van Diest PJ, van der Horst A, Smits AM, Burgering BM Cancer Res., 2008;68(18):7597-605. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Pin1 Ala2-Glu163 Accession # Q13526
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Pin1 is a peptidyl-prolyl isomerase (PPI) that targets phosphorylated Ser or Thr residues followed by a Pro (S/T-P). Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. Pin1 is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53,NF-kappa B, c-Jun, cyclin D1, and beta -catenin.
运输条件	Blue Ice
存放说明	4℃
参考文献	Checkpoint ICC / IHC Images: Pin1 NFkB Pathway p53 Pathway phospho-Serine/phospho-Threonine Binding Proteins

参考图片

Pin1 in Human Breast Cancer Tissue. Pin1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human/Mouse Pin1 by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pin1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.