| 货号 | MAB2417-SP |
| 别名 | BHLHB6; bHLHe21; Class B basic helix-loop-helix protein 6; Class E basic helix-loop-helix protein 21; helix-loop-helix protein, class B, 6; oligo1; oligodendrocyte lineage transcription factor 1; oligodendrocyte transcription factor 1; oligodendrocyte-specific bHLH transcription factor 1 | 全称 | Oligodendrocyte Lineage Gene 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human and mouse Olig1. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Olig2 or rhOlig3. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 116448 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Olig1 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Olig1 and Olig2, which have been implicated in oligodendrogenesis, areexpressed in the region of the ventral ventricular zone of lateembryonic spinal cord where oligodendrocyte progenitors appear. Olig3is transiently expressed in different types of progenitors of embryoniccentral nervous system and then disappears in the course ofdevelopment. In adult, expression of Olig3 is primarily detected inneural tissues. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Olig1 in Human Glioma. Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human Olig1 by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
