Human IFN-gamma MAb (Clone 25718) (25 UG)【科瑞奥博】

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Human IFN-gamma MAb (Clone 25718) (25 UG)

货号： MAB285-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB285-SP
别名	IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
全称	Interferon gamma
反应种属	Human
应用	Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human IFN-gamma.
使用方法	Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.02-0.06 µg/mL in the presence of 1 ng/mL Recombinant Human IFN‑ gamma.
来源	Monoclonal Mouse IgG_2A Clone # 25718
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Modulation of Stat-1 in Human Macrophages Infected with Different Species of Intracellular Pathogenic Bacteria J Immunol Res, 2016;2016(0):5086928. Species: Human Sample Type: Whole Cells Application: Neut Innate Invariant NKT Cell Recognition of HIV-1-Infected Dendritic Cells Is an Early Detection Mechanism Targeted by Viral Immune Evasion J Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: IHC - Fresh The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil Nat Immunol, 2015;16(3):318-25. Species: Human Sample Type: Whole Cells Application: Neut The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation. Authors: Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, McKinley C, Ahyi AN, Han L, Nguyen ET, Robertson MJ, Perumal NB, Tepper RS, Nutt SL, Kaplan MH Nat. Immunol., 2010;11(6):527-34. Species: Human Sample Type: Whole Cells Application: Neut Impaired development of human Th1 cells in patients with deficient expression of STAT4. Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH Blood, 2009;113(23):5887-90. Species: Human Sample Type: Whole Cells Application: Other: Cell differentiation In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation. Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F J. Clin. Invest., 2007;117(12):3868-3878. Species: Human Sample Type: Whole Cells Application: Neut Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia. Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA Exp. Hematol., 2006;34(10):1312-22. Species: Human Sample Type: Whole Cells Application: Neut Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Neut Peripheral expansion of circulating T-helper 1 cells predicts coronary endothelial dysfunction after cardiac transplantation. Authors: Methe H, Wiegand D, Welsch U, Meiser B, Nabauer M, Koglin J J. Heart Lung Transplant., 2005;24(7):833-40. Species: Human Sample Type: Whole Tissue Application: IHC IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E J. Immunol., 2004;173(2):1463-76. Species: Human Sample Type: Whole Cells Application: Neut Reprogramming of IL-10 activity and signaling by IFN-gamma. Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB J. Immunol., 2003;171(10):5034-41. Species: Human Sample Type: Whole Cells Application: Neut In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment. Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y Blood, 2003;102(12):4084-9. Species: Human Sample Type: Whole Cells Application: Neut Cutting edge: immune cells as sources and targets of the IL-10 family members? Authors: Wolk K, Kunz S, Asadullah K, Sabat R J. Immunol., 2002;168(11):5397-402. Species: Human Sample Type: Whole Cells Application: Functional Assay Decreased IL-15 may contribute to elevated IgE and acute inflammation in atopic dermatitis. Authors: Ong PY, Hamid QA, Travers JB, Strickland I, Al Kerithy M, Boguniewicz M, Leung DY J. Immunol., 2002;168(1):505-10. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IFN-gamma Gln24-Gln166 Accession # AAP20098.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

参考图片

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285). The ND₅₀ is typically 0.02-0.06 µg/mL.

IFN‑ gamma in Human PBMCs. IFN‑ gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB285) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.