Human CD36/SR-B3 MAb (Clone 255606) (25 UG)【科瑞奥博】

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Human CD36/SR-B3 MAb (Clone 255606) (25 UG)

货号： MAB19551-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB19551-SP
别名	CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; Collagen R; FAT; FATCHDS7; Fatty acid translocase; Glycoprotein IIIb; GP3Bthrombospondin receptor); GPIIIb; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3; SR-B3; Thrombospondin R; Thrombospondin receptor
全称	Scavenger Receptor Class B, Member 3
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunocytochemistry(3-25 µg/mL)
目标/特异性	Detects human CD36/SR‑B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD36 is observed.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunocytochemistry: 3-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	948 (Human); 12491 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD36/SR‑B3 Gly30-Asn439 Accession # P16671
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta ‑amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.
运输条件	Blue Ice
存放说明	4℃
参考文献	Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. Husemann, J. et al. (2002) Glia 40:195. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.

参考图片

CD36/SR‑B3 in U937 Human Cell Line. CD36/SR‑B3 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Rat Anti-Human CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB19551) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.