Human LRP-6 MAb (Clone 255302) (25 UG)【科瑞奥博】

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Human LRP-6 MAb (Clone 255302) (25 UG)

货号： MAB1505-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1505-SP
别名	ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6
全称	LDL Receptor-related Protein 6
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human LRP-6 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivitywith recombinant mouse (rm) LRP-6 and no cross-reactivity with recombinanthuman (rh) LRP-1, -4, -5, rhLRP-6 (intracellular domain), or rhLDL R isobserved.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 8-25 µg/mL
来源	Sterile PBS to a final concentration of 0.5 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4040 (Human); 16974 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds Proc Natl Acad Sci USA, 2016;113(21):E2945-54. Species: Human Sample Type: Whole Cells Application: Flow ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F Nature, 2012;485(7397):195-200. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human LRP-6 Ala20-Pro1368 Accession # O75581
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.5 mg/mL.
背景	LRP-6 (Low-density lipoprotein receptor-related protein 6) is a 200-210 kDa member of the LDLR family of proteins. It is widely expressed, and serves as a coreceptor for both Wnt and parathyroid hormone. In the Wnt system, LRP-6 associates with select Fzd multipass receptors; in the PTH system, LRP-6 complexes with PTH1R. Mature human LRP-6 is a 1594 amino acid (aa) type I transmembrane glycoprotein. It contains a 1351 aa extracellular region (aa 20-1370) plus a 220 aa cytoplasmic domain (aa 1394-1613). The cytoplasmic domain contains two palmitoylation sites, one ubiquitination residue, and multiple phosphorylation motifs.
运输条件	Blue Ice
存放说明	4℃
参考文献	Glucose Homeostasis ICC / IHC Images: LRP-6 Lipid/Cholesterol Metabolism Lipoprotein Receptors and Associated Proteins LRP Signaling Mini-review: Wnt Receptors & Pathways Obesity Wnt Family Wnt Receptors

参考图片

Detection of LRP-6 in MDA-
MB-231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human LRP‑6 Monoclonal Antibody (Catalog # MAB1505, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

LRP‑6 in DU145 Human Cell Line. LRP‑6 was detected in immersion fixed DU145 human prostate carcinoma cell line using Mouse Anti-Human LRP‑6 Monoclonal Antibody (Catalog # MAB1505) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.