| 货号 | MAB2097-SP |
| 别名 | DPC4; DPC4SMAD, mothers against DPP homolog 4 (Drosophila); hSMAD4; JIP; MAD homolog 4; MADH4; MADH4mothers against decapentaplegic homolog 4; mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD 4; SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila); SMAD, mothers against DPP homolog 4; Smad4 | 全称 | Mothers Against DPP Homolog 4 |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human Smad4 in direct ELISAs. |
| 使用方法 | Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4089 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Smad4 Pro139-Asp332 Accession # Q13485 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Mothers Against Decapentaplegic homolog 4 (Smad4) belongs to a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Upon signal-induced phosphorylation, Smad subunits associate with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Smad4 in HeLa Human Cell Line by Flow Cytometry. HeLa human cell line was stained with Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
