| 货号 | MAB21741-SP |
| 别名 | MGF; signal transducer and activator of transcription 5A; STAT5 | 全称 | Signal Transducer and Activator of Transcription 5 |
| 反应种属 | Human |
| 应用 | Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human STAT5a. This antibody does not cross-react with STAT5b. |
| 使用方法 | Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6776 (Human); 20850 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human STAT5a synthetic peptide SLDSRLSPPAGLFTSARGSLS Accession # NP_003143 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | STAT5a (Signal Transducer and Activator of Transcription-5a) is one of two closely related genes that belong to the STAT family of transcription factors. It is a 91 kDa cytosolic protein that contains an N-terminal domain (with an NES/Nuclear Export Signal) a coiled-coiled region (with an NLS/Nuclear Localization Signal) a DNA-binding site that recognizes a GAS (Gamma-interferon Activated Site) motif, an SH2 domain that allows for dimerization, and a C-terminal transactivation domain. STAT5a likely exists in a quiescent state as a cytoplasmic anti-parallel homodimer. Following activation of both tyrosine and non-tyrosine kinase membrane-bound receptors, STAT5a is phosphorylated, increasing its MW by some 5-6 kDa. Phosphorylated STAT5a will enter the nucleus as either a homodimer (or heterodimer with STAT5b), or as a complex with the intracellular domain of a tyrosine kinase receptor such as ErbB4. Once in the nucleus, STAT5a will form a homotetramer and bind either GAS or GAS-related sequences in gene regulatory regions. Notably, it is suggested that STAT5a may "cycle" through the nucleus without phosphorylation, a process that would seem to involve Importins alpha 3 and beta 1. STAT5a is related to STAT5b through gene duplication. They show 92% amino acid (aa) sequence identity, with the major differences existing over aa 45-56 and 773-794. The genes are not entirely redundant; STAT5a activates NDGR1 and SH2, while STAT5b regulates the Treg T cell genes, FoxP3 and CD25/IL-2R alpha. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of STAT5a in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT5a Monoclonal Antibody (Catalog # MAB21741, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. | |
STAT5a in K562 Human Cell Line. STAT5a was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human STAT5a Monoclonal Antibody (Catalog # MAB21741) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
