Human DLL1 MAb (Clone 251123) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human DLL1 MAb (Clone 251123) (25 UG)

货号： MAB18181-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB18181-SP
别名	delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; Drosophila Delta homolog 1; H-Delta-1
全称	Delta-like 1
反应种属	Human
应用	Immunohistochemistry(15-30 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human DLL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DLL3 or rhDLL4 is observed.
使用方法	Immunohistochemistry: 15-30 µg/mL Immunocytochemistry: 8-25 µg/mL
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	28514 (Human); 13388 (Mouse); 84010 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis. Authors: Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C Br. J. Dermatol., 2011;164(6):1228-34. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Hypoxia-induced Jagged2 promotes breast cancer metastasis and self-renewal of cancer stem-like cells. Authors: Xing F, Okuda H, Watabe M, Kobayashi A, Pai SK, Liu W, Pandey PR, Fukuda K, Hirota S, Sugai T, Wakabayshi G, Koeda K, Kashiwaba M, Suzuki K, Chiba T, Endo M, Mo YY, Watabe K Oncogene, 2011;30(39):4075-86. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders. Authors: Lasagni L, Ballerini L, Angelotti ML Stem Cells, 2010;28(9):1674-85. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human DLL1 Ser22-Gly540 Accession # AAG09716
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Delta-like protein 1 (DLL1) is a 90‑100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6‑11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. Karanu, F.N. et al. (2001) Blood 97:1960. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. Takahashi, Y. et al. (2003) Development 130:4259. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. Kiernan, A.E. et al. (2005) Development 132:4353. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749. Hozumi, K. et al. (2004) Nat. Immunol. 5:638. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454. Limbourg, A. et al. (2007) Circ. Res. 100:363. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

参考图片

DLL1 in HepG2 Human Cell Line. DLL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.