Human BLAME/SLAMF8 MAb (Clone 250014) (25 UG)【科瑞奥博】

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Human BLAME/SLAMF8 MAb (Clone 250014) (25 UG)

货号： MAB19072-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB19072-SP
别名	B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human BLAME/SLAMF8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SLAMF7 or rhSLAMF6 is observed.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	56833 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Immunol. Lett., 2011;134(2):167-73. Species: Human Sample Type: Whole Cells Application: Flow New B-cell CD molecules. Authors: Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P Immunol. Lett., 2011;134(2):104-12. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8 Ala23-Asp233 Accession # Q9P0V8
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%‑26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).
运输条件	Blue Ice
存放说明	4℃
参考文献	McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91. Veillette, A. (2006) Nat. Rev. Immunol. 6:56. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

参考图片

Detection of BLAME/SLAMF8 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were treated for with Recombinant Human IFN‑ gamma (Catalog # 285-IF) then stained with Mouse Anti-Human BLAME/SLAMF8 Monoclonal Antibody (Catalog # MAB19072, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0102B).