Human IL-12 p70 MAb (Clone 24910) (25 UG)【科瑞奥博】

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Human IL-12 p70 MAb (Clone 24910) (25 UG)

货号： MAB219-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB219-SP
别名	CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
全称	Interleukin 12 p70
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human IL-12 p70 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-12 p40 homodimer or rhIL-23 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑12 p70-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Stern, A. S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:6808. The Neutralization Dose (ND₅₀) is typically 0.3-0.9 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑12.
来源	Monoclonal Mouse IgG₁ Clone # 24910
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3592 (Human); 16159 (Mouse); 84405 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-1?, IL-4 and IL-12 control the fate of group 2 innate lymphoid cells in human airway inflammation in the lungs Authors: SM Bal, JH Bernink, M Nagasawa, J Groot, MM Shikhagaie, K Golebski, CM van Drunen, R Lutter, RE Jonkers, P Hombrink, M Bruchard, J Villaudy, JM Munneke, W Fokkens, JS Erjefält, H Spits, XR Ros Nat Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Bacillus Calmette-Guï¿½rin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses J Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes Authors: RB Jones, S Mueller, R OConnor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi PLoS Pathog, 2016;12(4):e1005545. Species: Human Sample Type: Whole Cells Application: Bioassay The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil Nat Immunol, 2015;16(3):318-25. Species: Human Sample Type: Whole Cells Application: Neut Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver. Authors: Jo J, Tan A, Ussher J, Sandalova E, Tang X, Tan-Garcia A, To N, Hong M, Chia A, Gill U, Kennedy P, Tan K, Lee K, De Libero G, Gehring A, Willberg C, Klenerman P, Bertoletti A PLoS Pathog, 2014;10(6):e1004210. Species: Human Sample Type: Whole Cells Application: Neut IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures. Authors: Blom L, Poulsen L J Immunol, 2012;189(9):4331-7. Species: Human Sample Type: Whole Cells Application: Neut Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S J. Immunol., 2009;183(12):8176-85. Species: Human Sample Type: Whole Cells Application: Bioassay Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus. Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V J. Exp. Med., 2009;206(4):793-805. Species: Human Sample Type: Whole Cells Application: Blocking Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay. Authors: Miller R, Sadhukhan R, Wu C J. Immunol. Methods, 2008;334(1):134-41. Species: Human Sample Type: Whole Cells Application: Neut Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke. Authors: Mian MF, Lauzon NM, Stampfli MR, Mossman KL, Ashkar AA J. Leukoc. Biol., 2007;83(3):774-84. Species: Human Sample Type: Whole Cells Application: Neut In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation. Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F J. Clin. Invest., 2007;117(12):3868-3878. Species: Human Sample Type: Whole Cells Application: Neut Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: Whole Cells Application: Neut Expression of toll-like receptors 2 and 4 in rheumatoid synovial tissue and regulation by proinflammatory cytokines interleukin-12 and interleukin-18 via interferon-gamma. Authors: Radstake TR, Roelofs MF, Jenniskens YM, van Riel PL, Barrera P Arthritis Rheum., 2004;50(12):3856-65. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The Toll-like receptor-2/6 agonist macrophage-activating lipopeptide-2 cooperates with IFN-gamma to reverse the Th2 skew in an in vitro allergy model. Authors: Weigt H, Muhlradt PF, Larbig M, Krug N, Braun A J. Immunol., 2004;172(10):6080-6. Species: Human Sample Type: Whole Cells Application: Neut NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs. Authors: Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L Blood, 2004;104(10):3267-75. Species: Human Sample Type: Whole Cells Application: ICC In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment. Authors: Durali D, de Goer de Herve MG, Giron-Michel J, Azzarone B, Delfraissy JF, Taoufik Y Blood, 2003;102(12):4084-9. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12 p70
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. Biologically active IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis gamma delta T Cells ICC / IHC Images: IL-12 p70 IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-12 Family IL-12: Cell Protection from UVB T Cell Cytokine Signaling Th1 Cells

参考图片

Cell Proliferation Induced by IL‑12 and Neutralization by Human IL‑12 p70 Antibody. Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB219). The ND₅₀ is typically 0.3-0.9 µg/mL.