Human HGF MAb (Clone 24612) (25 UG)【科瑞奥博】

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Human HGF MAb (Clone 24612) (25 UG)

货号： MAB294-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB294-SP
别名	deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
全称	Hepatocyte Growth Factor
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human HGF in Western blots.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize HGF-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 100 ng/mL Recombinant Human HGF.
来源	Monoclonal Mouse IgG₁ Clone # 24612
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3082 (Human); 15234 (Mouse); 403441 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis. Authors: Tyan SW, Kuo WH, Huang CK PLoS ONE, 2011;6(1):e15313. Species: Human Sample Type: Cell Lysates Application: WB A multiplex immunoassay for human adipokine profiling. Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E Clin. Chem., 2010;56(0):1320. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models. Authors: Coxon A, Rex K, Meyer S, Sun J, Sun J, Chen Q, Radinsky R, Kendall R, Burgess TL Mol. Cancer Ther., 2009;0(0):. Species: Human Sample Type: recombinant HGF Application: Neut alpha vbeta 6 Integrin promotes the invasion of morphoeic basal cell carcinoma through stromal modulation. Authors: Marsh D, Dickinson S, Neill GW, Marshall JF, Hart IR, Thomas GJ Cancer Res., 2008;68(9):3295-303. Species: Human Sample Type: Whole Cells Application: ICC Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O Int. J. Cancer, 2006;119(10):2313-21. Species: Human Sample Type: Whole Cells Application: Neut IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Cell Culture Supernates Application: Neut Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3. Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC Cancer Res., 2003;63(4):887-92. Species: Human Sample Type: Whole Cells Application: Flow Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts. Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M Transplant. Proc., 2000;32(5):987-9. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts. Authors: Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W PLoS ONE, 0;10(4):e0124283. Species: Human Sample Type: Whole Cells Application: Bioassay
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF Gln32-Ser728 Accession # P14210
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cytokines and Receptors in Angiogenesis EMT Induction Gametes and Fertilization Hepatic Endoderm HGF/SF Activation of Integrins ICC / IHC Images: HGF Integrins and Associated Molecules Pancreatic Endoderm Preeclampsia Semaphorins, Plexin Receptors, and Related Molecules

参考图片

Cell Proliferation Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF (Catalog # 294-HG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HGF (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294). The ND₅₀ is typically
0.1-0.3 µg/mL.