Human/Mouse SOX2 MAb (Clone 245610) (25 UG)【科瑞奥博】

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Human/Mouse SOX2 MAb (Clone 245610) (25 UG)

货号： MAB2018-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2018-SP
别名	ANOP3; MCOPS3; MGC2413; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2
全称	Transcription Factor SOX2
反应种属	Human/Mouse
应用	Western Blot(0.2 µg/mL) Simple Western(4 µg/mL) Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human and mouse SOX2 in Western blots.
使用方法	Western Blot: 0.2 µg/mL Simple Western: 4 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6657 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells Authors: Annabelle Congras Sci Rep, 2016;6(0):27059. Species: Procine Sample Type: Whole Cells Application: IHC - PFA fixed cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness Sci Rep, 2016;6(0):30742. Species: Mouse Sample Type: Whole Tissue Application: IHC - Fresh Glioma Cells in the Tumor Periphery Have a Stem Cell Phenotype PLoS ONE, 2016;11(5):e0155106. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A Stem Cells Transl Med, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: IHC Fresh Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion Stem Cells, 2016;0(0):. Species: Human Sample Type: Whole Tissue Application: IHC OCT-embedded EphA2 promotes infiltrative invasion of glioma stem cells in vivo through cross-talk with Akt and regulates stem cell properties. Authors: Miao, H, Gale, N W, Guo, H, Qian, J, Petty, A, Kaspar, J, Murphy, A J, Valenzuela, D M, Yancopoulos, G, Hambardzumyan, D, Lathia, J D, Rich, J N, Lee, J, Wang, B Oncogene, 2015;34(5):558-67. Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation. Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T Development, 2015;142(9):1628-38. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy. Authors: Yan K, Wu Q, Yan D, Lee C, Rahim N, Tritschler I, DeVecchio J, Kalady M, Hjelmeland A, Rich J Genes Dev, 2014;28(10):1085-100. Species: Human Sample Type: Whole Tissue Application: IF Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex. Authors: Minamide, Ryohei, Fujiwara, Kazushir, Hasegawa, Koichi, Yoshikawa, Kazuaki PLoS ONE, 2014;9(1):e84460. Species: Mouse Sample Type: tissue Application: IHC Paraffin-embedded Zrf1 is required to establish and maintain neural progenitor identity. Authors: Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L Genes Dev, 2014;28(2):182-97. Species: Mouse Sample Type: Whole Tissue Application: IHC - Frozen Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells. Authors: Izumikawa, Tomomi, Sato, Ban, Kitagawa, Hiroshi Sci Rep, 2014;4(0):3701. Species: Mouse Sample Type: Whole Cells Application: IF A single-cell and feeder-free culture system for monkey embryonic stem cells. Authors: Ono T, Suzuki Y, Kato Y, Fujita R, Araki T, Yamashita T, Kato H, Torii R, Sato N PLoS ONE, 2014;9(2):e88346. Species: Primate - Cynomolgus Monkey Sample Type: Whole Cells Application: ICC Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4. Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C J Immunol, 2014;192(1):523-32. Species: Human Sample Type: whole cells Application: IF Embryonic stem cell-based modeling of tau pathology in human neurons. Authors: Mertens J, Stuber K, Poppe D, Doerr J, Ladewig J, Brustle O, Koch P Am J Pathol, 2013;182(5):1769-79. Species: Human Sample Type: Whole Cells Application: ICC Fluorescence Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji PLoS ONE, 2012;7(9):e45435. Species: Human Sample Type: Whole Cells Application: ICC REST regulates oncogenic properties of glioblastoma stem cells. Authors: Kamal MM, Sathyan P, Singh SK, Zinn PO, Marisetty AL, Liang S, Gumin J, El-Mesallamy HO, Suki D, Colman H, Fuller GN, Lang FF, Majumder S Stem Cells, 2012;30(3):405-14. Species: Human Sample Type: Cell Lysates Application: WB Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells. Authors: Lathia JD, Gallagher J, Myers JT PLoS ONE, 2011;6(9):e24807. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Tissue-engineered vascular grafts form neovessels that arise from regeneration of the adjacent blood vessel. Authors: Hibino N, Villalona G, Pietris N FASEB J., 2011;25(8):2731-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells. Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS Mol. Ther., 2011;19(3):584-93. Species: Human Sample Type: Whole Cells Application: ICC Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells. Authors: West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL Stem Cells Dev., 2011;20(6):1079-88. Species: Human Sample Type: Whole Cells Application: ICC Subchronic peripheral neuregulin-1 increases ventral hippocampal neurogenesis and induces antidepressant-like effects. Authors: Mahar I, Tan S, Davoli MA, Dominguez-Lopez S, Qiang C, Rachalski A, Turecki G, Mechawar N PLoS ONE, 2011;6(10):e26610. Species: Mouse Sample Type: Whole Tissue Application: IHC Paternal recognition of adult offspring mediated by newly generated CNS neurons. Authors: Mak GK, Weiss S, Nat. Neurosci., 2010;13(6):753-8. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh Noninvasive imaging of endogenous neural stem cell mobilization in vivo using positron emission tomography. Authors: Rueger MA, Backes H, Walberer M, Neumaier B, Ullrich R, Simard ML, Emig B, Fink GR, Hoehn M, Graf R, Schroeter M J. Neurosci., 2010;30(18):6454-60. Species: Rat Sample Type: Whole Cells Application: ICC Epigenetic changes to human umbilical cord blood cells cultured with three proteins indicate partial reprogramming to a pluripotent state. Authors: Wong CJ, Casper RF, Rogers IM Exp. Cell Res., 2010;316(6):927-39. Species: Human Sample Type: Whole Cells Application: ICC Diagnostic utility of novel stem cell markers SALL4, OCT4, NANOG, SOX2, UTF1, and TCL1 in primary mediastinal germ cell tumors. Authors: Liu A, Cheng L, Du J, Peng Y, Allan RW, Wei L, Li J, Cao D Am. J. Surg. Pathol., 2010;34(5):697-706. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used. J. Cell. Biochem., 2009;106(5):867-77. Species: Human Sample Type: Whole Cells Application: ICC Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells. Authors: Neganova I, Zhang X, Atkinson S, Lako M Oncogene, 2009;28(1):20-30. Species: Human Sample Type: Cell Lysates Application: WB Toll-like receptor 3 is a negative regulator of embryonic neural progenitor cell proliferation. Authors: Lathia JD, Okun E, Tang SC, Griffioen K, Cheng A, Mughal MR, Laryea G, Selvaraj PK, Ffrench-Constant C, Magnus T, Arumugam TV, Mattson MP J. Neurosci., 2008;28(51):13978-84. Species: Mouse Sample Type: Whole Cells Application: ICC Efficient Serum-Free Derivation of Oligodendrocyte Precursors from Neural Stem Cell-Enriched Cultures. Authors: Rao RC, Boyd J, Padmanabhan R, Chenoweth JG, McKay RD Stem Cells, 2008;0(0):. Species: Mouse Sample Type: Whole Cells Application: ICC TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal. Authors: Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL Nat. Cell Biol., 2008;10(7):837-48. Species: Human Sample Type: Whole Cells Application: ICC Identification of SOX2 as a novel glioma-associated antigen and potential target for T cell-based immunotherapy. Authors: Schmitz M, Temme A, Senner V, Ebner R, Schwind S, Stevanovic S, Wehner R, Schackert G, Schackert HK, Fussel M, Bachmann M, Rieber EP, Weigle B Br. J. Cancer, 2007;96(8):1293-301. Species: Human Sample Type: Cell Lysates Application: WB Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue. Authors: Shin S, Sun Y, Liu Y, Khaner H, Svant S, Cai J, Xu QX, Davidson BP, Stice SL, Smith AK, Goldman SA, Reubinoff BE, Zhan M, Rao MS, Chesnut JD Stem Cells, 2007;25(5):1298-306. Species: Human Sample Type: Whole Cells Application: ICC Glioblastoma-derived stem cell-enriched cultures form distinct subgroups according to molecular and phenotypic criteria. Authors: Gunther HS, Schmidt NO, Phillips HS, Kemming D, Kharbanda S, Soriano R, Modrusan Z, Meissner H, Westphal M, Lamszus K Oncogene, 2007;27(20):2897-909. Species: Human Sample Type: Whole Cells Application: Flow Identification of a neuron-specific human gene, KIAA1110, that is a guanine nucleotide exchange factor for ARF1. Authors: Hattori Y, Ohta S, Hamada K, Yamada-Okabe H, Kanemura Y, Matsuzaki Y, Okano H, Kawakami Y, Toda M Biochem. Biophys. Res. Commun., 2007;364(4):737-42. Species: Human Sample Type: Whole Cells Application: ICC Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage. Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC Stem Cells, 2007;25(6):1511-20. Species: Human Sample Type: Whole Cells Application: ICC Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O Stroke, 2006;37(9):2361-7. Species: Rat Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human SOX2 Gly135-Met317 Accession # P48431
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
运输条件	Blue Ice
存放说明	4℃
参考文献	Graham, V. et al. (2003) Neuron 39:749. Avilion, A.A. et al. (2003) Genes Dev. 17:126. Kishi, M. et al. (2000) Development 127:791. Yuan, H. et al. (1995) Genes Dev. 9:2635. Uwanogho, D. et al. (1995) Mech. Dev. 49:23. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

参考图片

E‑Cadherin and SOX2 in BG01V Human Stem Cells. E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse Anti-Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (red; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human SOX2 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SOX2 in BG01V Human Embryonic Stem Cells. SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human SOX2 by Simple Western^TM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.