| 货号 | MAB2024-SP |
| 别名 | hPROML2; MGC138714; PROM2; PROM-2; prominin 2; prominin-2; Prominin-like protein 2; prominin-related protein; PROML2 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human Prominin 2. Stains human Prominin 2-transfected cells but not irrelevant transfectants. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 150696 (Human); 192212 (Mouse); 192211 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human Prominin 2 Ala27-Leu834 Accession # AAM10541 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Prominin 2 is a pentaspan membrane glycoprotein predominantly expressed in neuroepithelial cells, hematopoietic stem cells and epithelial cells of the adult kidney and digestive tract (1, 2). Prominin 2 is a 112-kDa glycoprotein structurally related to prominin 1 (CD133). The amino acid identity between prominin 1 and prominin 2 is low (<30%), but they exhibit some redundant functions. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Prominin 2 in HepG2 Human Cell Line. Prominin 2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Prominin 2 Monoclonal Antibody (Catalog # MAB2024) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
