Human Serpin E1/PAI-1 MAb (Clone 242816) (25 UG)【科瑞奥博】

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Human Serpin E1/PAI-1 MAb (Clone 242816) (25 UG)

货号： MAB1786-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1786-SP
别名	Endothelial plasminogen activator inhibitor; Nexin; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1
全称	Plasminogen Activator Inhibitor
反应种属	Human
应用	Western Blot,Simple Western,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed.
使用方法	Western Blot: 1 µg/mL Simple Western: 2.5 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) inhibition of Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) cleavage of the fluorogenic peptide substrate Z-GGR-AMC (100 µM). The Neutralization Dose (ND₅₀) is typically 0.3 µg/mL.
来源	Monoclonal Mouse IgG₁ Clone # 242816
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5054 (Human); 18787 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Human CD4- 8- T cells are a distinctive immunoregulatory subset. Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ FASEB J., 2010;24(7):2558-66. Species: Human Sample Type: Whole Cells Application: Neut A multiplex immunoassay for human adipokine profiling. Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E Clin. Chem., 2010;56(0):1320. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin E1/PAI-1 Met1-Pro402 Accession # P05121
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI-1 is the principal inhibitor of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI-1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N-terminal signal peptide (6, 7). PAI-1 may exist in one of two possible conformations, designated as active or latent (8).
运输条件	Blue Ice
存放说明	4℃
参考文献	Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293. Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545. Duffy, M.J. (2002) Clin. Chem. 48:1194. Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196. Pannekoek, H. et al. (1986) EMBO J. 5:2539. Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673. Wang, Z. et al. (1996) Biochemistry 35:16443. Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309.

参考图片

Detection of Human Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin E1/PAI‑1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization of Serpin E1/
PAI‑1 Activity by Human Serpin E1/PAI‑1 Antibody.

Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) activity is measured in the presence of Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) that has been preincubated with increasing concentrations of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786). The ND₅₀ is typically 0.3 µg/mL.

Detection of Human Serpin E1/PAI‑1 by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 54 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Serpin E1/PAI‑1 Monoclonal Antibody (Catalog # MAB1786). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.