Human/Mouse HIF-1 alpha MAb (Clone 241812) (25 UG)【科瑞奥博】

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Human/Mouse HIF-1 alpha MAb (Clone 241812) (25 UG)

货号： MAB1935-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1935-SP
别名	ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF-1 alpha; HIF1A; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1HIF1-ALPHA; PAS domain-containing protein 8; PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
全称	Hypoxia-inducible Transcription Factor 1 alpha
反应种属	Human/Mouse
应用	Immunohistochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Recognizes human and mouse HIF-1 alpha in direct ELISAs.
使用方法	Immunohistochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3091 (Human); 15251 (Mouse); 29560 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R Proc Natl Acad Sci U S A, 2015;112(9):E957-65. Species: Mouse Sample Type: Whole Cells Application: Flow Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model. Authors: Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S Cancer Res., 2007;67(12):5789-97. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human HIF-1 alpha Arg575-Asn826 Accession # Q16665.1
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
运输条件	Blue Ice
存放说明	4℃
参考文献	Basic Helix-Loop-Helix (bHLH) Transcription Factors Cancer Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators HIF Transcription Factors HIF-1 & Vascular Development ICC / IHC Images: HIF-1 alpha Interactive Pathway: HIF Enhancers Interactive Pathway: HIF Repressors Intracellular Factors in Inflammation Intracellular Signaling Molecules in Angiogenesis Notch Pathway ROS and Stress Resistance Transcription Factors and Regulators Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

HIF‑1 alpha in Human Kidney Cancer Tissue. HIF‑1 alpha was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Mouse Anti-Human/Mouse HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1935) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.