Human/Mouse/Rat HIF-1 alpha MAb (Clone 241809) (25 UG)【科瑞奥博】

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Human/Mouse/Rat HIF-1 alpha MAb (Clone 241809) (25 UG)

货号： MAB1536-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1536-SP
别名	ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF-1 alpha; HIF1A; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1HIF1-ALPHA; PAS domain-containing protein 8; PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
全称	Hypoxia-inducible Transcription Factor 1 alpha
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL) Simple Western(1 µg/mL) Immunoprecipitation(1-3 µg/500 µg cell lysate)
目标/特异性	Detects human, mouse, and rat HIF-1 alpha.
使用方法	Western Blot: 1 µg/mL Simple Western: 1 µg/mL Immunoprecipitation: 1-3 µg/500 µg cell lysate
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3091 (Human); 15251 (Mouse); 29560 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity Eur J Immunol, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Flow Evolutionary conserved regulation of HIF-1beta by NF-kappaB. Authors: van Uden P, Kenneth NS, Webster R, Muller HA, Mudie S, Rocha S PLoS Genet., 2012;7(1):e1001285. Species: Human Sample Type: Cell Lysates Application: WB The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice. Authors: Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC J. Clin. Invest., 2010;120(3):827-39. Species: Mouse Sample Type: Tissue Homogenates Application: WB Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs. Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X Biochem. Biophys. Res. Commun., 2010;401(4):509-15. Species: Mouse Sample Type: Cell Lysates Application: WB Oxygen tension regulates preosteocyte maturation and mineralization. Authors: Zahm AM, Bucaro MA, Srinivas V, Shapiro IM, Adams CS Bone, 2008;43(1):25-31. Species: Mouse Sample Type: Cell Lysates Application: WB CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma. Authors: Monnier Y, Farmer P, Bieler G, Imaizumi N, Sengstag T, Alghisi GC, Stehle JC, Ciarloni L, Andrejevic-Blant S, Moeckli R, Mirimanoff RO, Goodman SL, Delorenzi M, Ruegg C Cancer Res., 2008;68(18):7323-31. Species: Human Sample Type: Cell Lysates Application: WB Early induction of neuronal lipocalin-type prostaglandin D synthase after hypoxic-ischemic injury in developing brains. Authors: Taniguchi H, Mohri I, Okabe-Arahori H, Kanekiyo T, Kagitani-Shimono K, Wada K, Urade Y, Nakayama M, Ozono K, Taniike M Neurosci. Lett., 2007;420(1):39-44. Species: Mouse Sample Type: Whole Tissue Application: IHC Nucleus pulposus cells express HIF-1alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. Authors: Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM J. Cell. Biochem., 2006;98(1):152-9. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human HIF-1 alpha Arg575-Asn826 Accession # Q16665.1
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
运输条件	Blue Ice
存放说明	4℃
参考文献	Basic Helix-Loop-Helix (bHLH) Transcription Factors Cancer Stem Cell Transcription Factors Hematopoietic Stem Cell Transcription Factors and Regulators HIF Transcription Factors HIF-1 & Vascular Development ICC / IHC Images: HIF-1 alpha Interactive Pathway: HIF Enhancers Interactive Pathway: HIF Repressors Intracellular Factors in Inflammation Intracellular Signaling Molecules in Angiogenesis Notch Pathway ROS and Stress Resistance Transcription Factors and Regulators Transcription Factors in the Akt Pathway Transcriptional Regulators in Allergy and Asthma

参考图片

Detection of Human, Mouse, and Rat HIF‑1 alpha by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 μM CoCl₂ for 8 hours. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human HIF‑1 alpha by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O₂), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 116 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.