| 货号 | MAB2479-SP |
| 别名 | persephin; PSP; PSPN |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse Persephin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) AgRP, rmArtemin, rmCripto, recombinant drosophila DPP, recombinant human DPP-6, rhGDNF, rhLAP, rmMIS, rmNodal, rhTGF‑ alpha, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2, rhTGF-beta 3, or recombinant amphibian TGF-beta 5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5623 (Human); 19197 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse Persephin Ala61-Gly156 Accession # O70300 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Persephin is a secreted protein belonging to the glial cell line‑derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38‑46% amino acid identity with family members GDNF, neurturin and artemin. Persephin is expressed at very low levels in most tissues (1). The 10‑12 kDa mature protein contains several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked homodimer. Mature mouse persephin shares 94%, 81%, 79% and 76% amino acid sequence identity with rat, human, bovine and canine persephin, respectively. Like other GDNF family members, persephin acts through engagement of a glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development (1). These effects are correlated with the expression patterns of its specific receptor, GFR alpha 4, and RET (2, 3). Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system and include GPI-linked, transmembrane and soluble forms (3, 4). In vitro, persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET (2, 5). This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members (6). Disruption of the persephin gene results in mice that are morphologically normal but have more damage and less effective repair after a central nervous system insult simulating a stroke. Microinjection of persephin prior to treatment protects against damage in both wild-type and mutant mouse brains, but surprisingly, high doses of persephin are detrimental (7). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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