Human/Mouse Oct-3/4 MAb (Clone 240408) (25 UG)【科瑞奥博】

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Human/Mouse Oct-3/4 MAb (Clone 240408) (25 UG)

货号： MAB1759-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1759-SP
别名	Otf3g; Otf4; Pou5f1
全称	Octamer-binding Protein 3/4
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5460 (Human); 18999 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis. Authors: Cortes-Dericks, Lourdes, Yazd, Ehsan Fa, Mowla, Seyed J, Schmid, Ralph A, Karoubi, Golnaz Anticancer Res, 2013;33(12):5365-73. Species: Human Sample Type: Cell Lysates Application: WB The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells. Authors: Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K Stem Cells Int, 2012;2012(0):875059. Species: Human Sample Type: Whole Cells Application: ICC Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells. Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS Mol. Ther., 2011;19(3):584-93. Species: Human Sample Type: Whole Cells Application: ICC Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. Authors: Angel M, Yanik MF, PLoS ONE, 2010;5(7):e11756. Species: Human Sample Type: Whole Cells Application: ICC A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Authors: Klim JR, Li L, Wrighton PJ Nat. Methods, 2010;7(12):989-94. Species: Human Sample Type: Whole Cells Application: ICC Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Authors: Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA Cell Stem Cell, 2009;4(4):359-69. Species: Mouse Sample Type: Whole Cells Application: ICC Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays. Authors: Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP Stem Cells, 2008;26(10):2586-94. Species: Mouse Sample Type: Whole Cells Application: ICC Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ Stem Cells, 2008;26(6):1436-43. Species: Human Sample Type: Whole Cells Application: ICC Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis. Authors: Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S Cancer Res., 2008;68(16):6533-40. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Stem Cells, 2007;25(2):490-9. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) Accession # Q01860
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613. Scholer, H.R. et al. (1989) EMBO J. 8:2543. Rosner, M.H. et al. (1990) Nature 345:686. Niwa, H. et al. (2000) Nat. Genet. 24:372.

参考图片

Detection of Human Oct-3/4 by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Oct-3/4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Oct‑3/4 in BG01V Human Embryonic Stem Cells.

Oct‑3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using Rhodamine Red-coupled anti-rat IgG (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.