Human CCL4/MIP-1 beta MAb (Clone 24006) (25 UG)【科瑞奥博】

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Human CCL4/MIP-1 beta MAb (Clone 24006) (25 UG)

货号： MAB271-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB271-SP
别名	ACT2; ACT-2; AT744.1; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2
反应种属	Human
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP‑1 alpha and no cross-reactivity with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3 is observed.
使用方法	Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL4/MIP‑1 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.3-1.2 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL4/MIP‑1 beta.
来源	Monoclonal Mouse IgG_2B Clone # 24006
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. HIV-1-induced impairment of dendritic cell cross talk with gammadelta T lymphocytes. Authors: Cardone M, Ikeda K, Varano B, Gessani S, Conti L J Virol, 2015;89(9):4798-808. Species: Human Sample Type: Whole Cells Application: Neut Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer. Authors: Kageyama S, Ikeda H, Miyahara Y, Imai N, Ishihara M, Saito K, Sugino S, Ueda S, Ishikawa T, Kokura S, Naota H, Ohishi K, Shiraishi T, Inoue N, Tanabe M, Kidokoro T, Yoshioka H, Tomura D, Nukaya I, Mineno J, Takesako K, Katayama N, Shiku H Clin Cancer Res, 2015;21(10):2268-77. Species: Human Sample Type: Whole Cells Application: ELISpot Development Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Authors: Franciszkiewicz K, Le Floch A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Cancer Res., 2009;69(15):6249-55. Species: Human Sample Type: Whole Cells Application: ICC Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes. Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC Clin. Immunol., 2008;128(1):57-65. Species: Human Sample Type: Serum Application: ELISA Development Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua. Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ J. Pathol., 2008;214(3):328-36. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P AIDS, 2007;21(10):1263-72. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling. Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M J. Clin. Invest., 2007;117(12):3664-3672. Species: Human Sample Type: Whole Cells Application: Neut Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Arthritis Rheum., 2006;54(7):2096-108. Species: Human Sample Type: Cell Culture Supernates Application: Immunodepletion Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: blister fluid Application: Neut Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression. Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B J. Virol., 2006;80(1):236-45. Species: Primate - Macaca fascicularis (Crab-eating or Cynomolgus macaque) Sample Type: Cell Culture Supernates Application: ELISA Development Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells. Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P J. Leukoc. Biol., 2004;75(3):504-14. Species: Human Sample Type: Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta Ala24-Asn92 Accession # P13236
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Cytokines Suppress HIV Replication ICC / IHC Images: CCL4/MIP-1 beta Infectious Diseases Inflammatory Mediators Macrophage Activation Markers Neuroinflammation Regulatory T Cells (Tregs)

参考图片

Chemotaxis Induced by CCL4/MIP‑1 beta and Neutralization by Human CCL4/MIP‑1 beta Antibody. Recombinant Human
CCL4/MIP‑1 beta (Catalog #
271-BME) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human
CCL4/MIP‑1 beta (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human
CCL4/MIP‑1 beta Monoclonal Antibody (Catalog # MAB271). The ND₅₀ is typically
0.3-1.2 µg/mL.

Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monoctyes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP‑1 beta Monoclonal Antibody (Catalog # MAB271) or (B) Mouse IgG_2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.