Human ADAM15 MAb (Clone 23G9) (25 UG)【科瑞奥博】

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Human ADAM15 MAb (Clone 23G9) (25 UG)

货号： MAB935-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB935-SP
别名	ADAM 15; ADAM metallopeptidase domain 15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin
全称	A Disintegrin and Metalloprotease-like Domain 15
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunoprecipitation,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry
目标/特异性	Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross‑reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8751 (Human); 11490 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression. Authors: Chatterjee V, Beard R, Reynolds J, Haines R, Guo M, Rubin M, Guido J, Wu M, Yuan S PLoS ONE, 2014;9(10):e110286. Species: Human Sample Type: Whole Cells Application: Flow ADAM15 expression is downregulated in melanoma metastasis compared to primary melanoma. Authors: Ungerer C, Doberstein K, Burger C, Hardt K, Boehncke WH, Bohm B, Pfeilschifter J, Dummer R, Mihic-Probst D, Gutwein P Biochem. Biophys. Res. Commun., 2010;401(3):363-9. Species: Human Sample Type: Whole Cells Application: Flow Homeostatic effects of the metalloproteinase disintegrin ADAM15 in degenerative cartilage remodeling. Authors: Bohm BB, Aigner T, Roy B, Brodie TA, Blobel CP, Burkhardt H Arthritis Rheum., 2005;52(4):1100-9. Species: Mouse Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15 Asp207-Thr696 Accession # Q13444
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	ADAM15 belongs to a family of transmembrane proteins that containdisintegrin and metalloprotease domains. Members of this family havebeen implicated in cell adhesion via integrin binding and shedding ofcell surface molecules.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling Cell to Cell Adhesion Disassembly During EMT Extracellular Matrix Remodeling and Cell Migration During EMT ICC / IHC Images: ADAM15 Metalloproteases and Regulators Mini-review: ADAMs

参考图片

ADAM15 in MCF‑7 Human Cell Line. ADAM15 was detected in immersion fixed MCF‑7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ADAM15 in Human Prostate Cancer Tissue. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at
4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.