| 货号 | MAB2079-SP |
| 别名 | dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC 2.7.12.2; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) | 全称 | Mitogen-activated Protein Kinase Kinase 1 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat MEK1 in Western blots. In Western blots, no crossreactivity with recombinant human MEK2 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5604 (Human); 26395 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human MEK1 Lys2-Val393 (Ser218Asp, Ser222Asp) Accession # Q02750 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Mitogen-Activated Protein Kinase Kinase 1 (MEK1 or MAP2K1), also known as MKK1, belongs to the family of STE kinases. Both MEK1 and the related MEK2 aredual‑specificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. Activation of MEK1 by Raf occurs through phosphorylation at S218 and S222. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat MEK1 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat MEK1 Monoclonal Antibody (Catalog # MAB2079) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK1 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
