| 货号 | MAB1904-SP |
| 别名 | CLEC11a; CLECSF3; LSLCL | 全称 | Stem Cell Growth Factor/C-type Lectin Domain Family 11, Member A |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human SCGF/CLEC11a in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse SCGF-beta is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6320 (Human); 20256 (Mouse); 29313 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Proteomic detection of a large amount of SCGFalpha in the stroma of GISTs after imatinib therapy. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human SCGF/CLEC11a Gly19-Phe245 Accession # BAA21499 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Stem Cell Growth Factor (SCGF) is a secreted sulfated glycoprotein that belongs to the C-type lectin superfamily (CLEC11a) and is expressed primarily in hematopoietic tissues. It functions as a growth factor for primitive hematopoietic progenitor cells. SCGF-beta is a truncated variant of SCGF-alpha that has aa 78 amino acid deletion and lacks a functional calcium-dependent carbohydrate-recognition domain. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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SCGF/CLEC11a in THP-1 Human Cell Line. SCGF/CLEC11a was detected in immersion fixed IFN gamma activated THP-1 human acute monocytic leukemia cell line using 10 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
SCGF/CLEC11a in Human Spleen. SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
