| 货号 | MAB16991-SP |
| 别名 | antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide); Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6 |
| 反应种属 | Human/Equine |
| 应用 | Flow Cytometry(0.25 µg/106cells) Immunohistochemistry(5-25 µg/mL) Immunocytochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects human CD11b/Integrin alpha M in direct ELISAs. |
| 使用方法 | Flow Cytometry: 0.25 µg/106cells Immunohistochemistry: 5-25 µg/mL Immunocytochemistry: 8-25 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3684 (Human); 16409 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M Phe17-Asn1105 Accession # NP_001139280 |
| 生物活性 | Human, Equine |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The Integrin alpha M subunit (CD11b), associates with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimeric Integrin CD11b/CD18, also known as Mac-1 and CR-3. Upon activation, CD11b/CD18 is expressed on granulocytes, monocytes, a subset of NK cells and activated lymphocytes. Integrin CD11b/CD18 functions as a receptor for complement fragment iC3b, ICAM-1 (CD54), ICAM-2 (CD102) and fibrinogen to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1‑3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of CD11b/Integrin alpha M in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Equine CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16991, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). | |
CD11b/Integrin alpha M in Human PBMCs. CD11b/Integrin alpha M was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Equine CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16991) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
CD11b/Integrin alpha M in Human Brain. CD11b/Integrin alpha M was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using Mouse Anti-Human/Equine CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16991). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of microglia. Tissue was co-stained with using a Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-Purified Polyclonal Anitbody (Catalog # AF7947) and an Alexa Fluor® 488-conjugated Donkey Anti-Sheep IgG Secondary Antibody (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
