货号 | MAB1864-SP |
别名 | CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5 |
反应种属 | Human |
应用 | Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(8-25 µg/mL) |
目标/特异性 | Detects human Integrin alpha 5/CD49e. |
使用方法 | Flow Cytometry: 2.5 µg/106cells Adhesion Activation: The adhesion of K562 human chronic myelogenous leukemia cells (5 x 104 cells/well) to immobilized Bovine Fibronectin (Catalog # 1030-FN, 10 µg/mL, 100 µL/well) was maximally enhanced (2-2.5 fold) by pre-treatment of cells with 500 ng/mL of the antibody. Immunocytochemistry: 8-25 µg/mL |
来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3678 (Human); 16402 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering. | |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e Phe42-Tyr995 Accession # P08648 |
内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | The Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
|