| 货号 | MAB5627-SP |
| 别名 | testis expressed 11; testis expressed sequence 11; testis-expressed sequence 11 protein; Tex11; TGC1; TSGA3 | 全称 | Zinc Transporter Protein 4 Homolog |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects mouse ZIP4H/Tex11 in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56159 (Human); 83558 (Mouse); 501588 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse ZIP4H/Tex11 Met768-Leu947 Accession # Q14AT2 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | ZIP4 homolog (ZIP4H), also known as testis expressed sequence 11 (Tex11) is a 947 amino acid (aa) X chromosome-encoded protein that is selectively expressed in primordial male germ cells. It associates with Nbs1 of the Mre11 protein complex which results in localization to areas of synaptonemal contact between meiotic chromosomes. During meiosis, ZIP4H promotes sister chromatid synapsis and crossover in response to programmed double stranded DNA breaks. ZIP4H deficiency leads to increased spermatocyte apoptosis and male sterility. Alternate splicing in mouse generates an additional isoform with a substitution (aa 691‑707) and truncation (aa 708‑947) at the C-terminus. Within aa 768‑947, mouse ZIP4H shares 44% and 73% aa sequence identity with human and rat ZIP4H, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse ZIP4H/Tex11 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line non-transfected or transfected with recombinant mouse ZIP4H or recombinant human ZIP4H. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse ZIP4H/Tex11 Monoclonal Antibody (Catalog # MAB5627) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for ZIP4H/Tex11 was detected at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
